Printing metallic inks on devices

Capturing cells on GO functionalized with proteins

Printing protein pattern with microchannel probe on carbon film of TEM grid

Printing biotin-azide on polymer brush for capturing sensitized bacteria

Printing proteins on nanoneedles with microchannel probe

Accessing AFM cantilever with microchannel probe to deposit proteins for cell adhesion

3D lipid metallization

A fluid phase phospholipid ink was used as a building block for structuring with dip-pen nanolithography. Following a bioinspired approach that relies on ink-spreading inhibition, two processes are presented to build 2D and 3D metallic structures. Serum albumin, a widely used protein with an innate capability to bind to lipids, is the key in both processes. Covering the sample with BSA anchors lipids on the substrate, which allows the creation of highly stable 3D lipid-based scaffolds.

ssDNA arrays

ssDNA arrays used as high-throughput platform of localized catalytic nanocompartments (CNC) that allow for multistep signal propagation

Protein arrays on GO

Protein microarrays on GO coatings prepared for cell culture experiments.

Surface Modification by Click Reactions

The thiol-terminated surface is spotted by microchannel cantilever spotting with different fluorescent and nonfluorescent inks containing cycloalkyne (dibenzocyclooctyne (DBCO)) or alkene (maleimide) groups for fabricating biosensors, appropriate for protein detection and other biomedical/biological applications.

Lipid arrays with functional headgroups

Application of Lipid-DPN for the generation of arrays of the ligand 2,4-dinitrophenyl[1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[6-[(2,4-dinitrophenyl)amino]hexanoyl] (DNP)] onto glass surfaces as a model system for detection of allergen-specific Immunoglobin E (IgE) antibodies and for mast cell activation profiling.

2D materials

Graphene spotted on the glass slide with Molecular Printer using micro-contact spotting.

Lipid reactors on polymers

The embedded lipid arrays show high stability against washing steps, while still being accessible for protein and antibody binding. HEMA-EDMA polymer was used to generate functional arrays of selected lipid–protein pairs applied in previous settings: Biotin-Cap-PE and Streptavidin labeled with Cy3 dye (STV-Cy3) as a simple protein model; and DNP-cap-PE with anti-DNP IgE as a model for allergen/antibody recognition.

DNA arrays

DNA-NH2 printed on GPTMS coated slides with Molecular Printer using micro-contact printing.

LOC

Lab-on-chip device functionalized with Molecular Printer using fibronectin before applying it in cell culture.

Polymerization

Cationic ring-opening polymerization of 2-alkyl-2-oxazolines with Molecular Printer using micro-contact spotting.

Oligonucleotide arrays

Covalent immobilization of oligonucleotide arrays with up to five different components with Molecular Printer using polymeric stamp.

Nanofibers

The locally defined growth of carbon nanofibers with lambda after deposition of eposition of catalytic salt NiCl2 · 6H2O with Molecular Printer using micro-contact spotting.

Cell arrays

Multiprotein patterns of fibronectin, laminin and ephirin on the glass slide fabricated using polymeric stamps.

Lipid arrays

Various lipid inks printed on the glass slide with Molecular Printer using cantilever tip arrays.

Protein network

Covalent immobilization of proteins with Molecular Printer using micro-contact stamp.

Optical sensors

Passive photonic whispering gallery mode microresonators functionalized with cantilever tip arrays.

Protein dots

Covalent immobilization of proteins with Molecular Printer using micro-contact stamp.

Multi-cell array

ultiprotein patterns of fibronectin, laminin and ephirin on the glass slide fabricated using polymeric stamps for culturing various cell types simultaneously.